Rabbit METTL14 Polyclonal Antibody | anti-METTL14 antibody

METTL14 Antibody (N-Term)

Cat. Num. AAA9231311

• Gene Names: METTL14; hMETTL14
• Reactivity: Human, Mouse; Predicted: Bovine
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: METTL14; Polyclonal Antibody; METTL14 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); N6-adenosine-methyltransferase subunit METTL14; 2.1.1.62; Methyltransferase-like protein 14; KIAA1627; anti-METTL14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This METTL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the human region of human METTL14.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 456

• Applicable Applications for anti-METTL14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-METTL14 Antibody (N-Term) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: A431 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-METTL14 antibody

The METTL3-METTL14 heterodimer forms a N6- methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA- binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27373337, PubMed:27281194). N6- methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).

Product Categories/Family for anti-METTL14 antibody

Crown Antibodies; Primary Antibodies; Cell Biology

References

Nagase T.,et al.DNA Res. 7:273-281(2000). Nakajima D.,et al.DNA Res. 9:99-106(2002). Ota T.,et al.Nat. Genet. 36:40-45(2004). Hillier L.W.,et al.Nature 434:724-731(2005). Mural R.J.,et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 24308265
• NCBI GeneID: 57721
• NCBI Accession #: NP_066012.1
• NCBI GenBank Nucleotide #: NM_020961.3
• UniProt Accession #: Q9HCE5; Q969V2; A6NIG1
• Molecular Weight: 52150
• NCBI Official Full Name: N6-adenosine-methyltransferase non-catalytic subunit
• NCBI Official Synonym Full Names: methyltransferase like 14
• NCBI Official Symbol: METTL14
• NCBI Official Synonym Symbols: hMETTL14
• NCBI Protein Information: N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14
• UniProt Protein Name: N6-adenosine-methyltransferase non-catalytic subunit
• Protein Family: N6-adenosine-methyltransferase
• UniProt Gene Name: METTL14
• UniProt Synonym Gene Names: hMETTL14

Uniprot Description

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells ().