Canine Bone Morphogenetic Protein 6 ELISA Kit | BMP6 elisa kit

Canine Bone Morphogenetic Protein 6 ELISA Kit

Cat. Num. AAA740849

• Gene Names: BMP6; VGR; VGR1
• Reactivity: Canine
• Synonyms: Bone Morphogenetic Protein 6; Canine Bone Morphogenetic Protein 6 ELISA Kit; BMP6 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This BMP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BMP6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP6 elisa kit

Principle of the Assay: BMP6 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BMP6. Standards or samples are then added to the microtiter plate wells and BMP6 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BMP6 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for BMP6 are added to each well to "sandwich" the BMP6 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BMP6 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BMP6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502425
• NCBI GeneID: 654
• NCBI Accession #: NP_001709.1
• NCBI GenBank Nucleotide #: NM_001718.4
• Molecular Weight: 57,226 Da
• NCBI Official Full Name: bone morphogenetic protein 6 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 6
• NCBI Official Symbol: BMP6
• NCBI Official Synonym Symbols: VGR; VGR1
• NCBI Protein Information: bone morphogenetic protein 6; BMP-6; VGR-1; VG-1-R; VG-1-related protein; Vg1-related sequence; vegetal-related (TGFB related) cytokine; vegetal related growth factor (TGFB-related)
• UniProt Protein Name: Bone morphogenetic protein 6
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP6
• UniProt Synonym Gene Names: VGR; BMP-6; VG-1-R; VGR-1
• UniProt Entry Name: BMP6_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP6: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p24-p23

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; protein heterodimerization activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: cellular iron ion homeostasis; response to glucocorticoid stimulus; regulation of apoptosis; BMP signaling pathway; response to magnesium ion; positive regulation of endothelial cell differentiation; positive regulation of aldosterone biosynthetic process; male genitalia development; inflammatory response; kidney development; response to iron ion; skeletal development; endochondral ossification; response to retinoic acid; positive regulation of bone mineralization; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; eye development; positive regulation of protein secretion; cartilage development; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of endothelial cell proliferation; response to activity; positive regulation of neuron differentiation; positive regulation of epithelial cell proliferation; growth