SDS-PAGE
MANF / ARMET recombinant protein
Recombinant Mouse MANF / ARMET Protein (His tag)
Gene Names
Manf; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
Purity
> 90 % as determined by SDS-PAGE
Synonyms
MANF / ARMET; Recombinant Mouse MANF / ARMET Protein (His tag); 3230402M22Rik; AA407711; AA408789; AA673178; Armet; D17914; D18Mgi17; MANF / ARMET recombinant protein
Host
Human Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
179
Application Notes
The secreted recombinant mouse MANF comprises 169 amino acids and has a calculated molecular mass of 19.6 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Leu 22
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for MANF / ARMET recombinant protein
Background: Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF and ARMET, is a secreted protein which belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue type s.
Description: A DNA sequence encoding the mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,374 Da
NCBI Official Full Name
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Synonym Full Names
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Symbol
Manf
NCBI Official Synonym Symbols
Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
NCBI Protein Information
mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
UniProt Gene Name
Manf
UniProt Synonym Gene Names
Armet
Uniprot Description
ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9 F1|9 57.98 cM
Cellular Component: endoplasmic reticulum; extracellular space; nucleus
Biological Process: neurite development; vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure
Research Articles on MANF / ARMET
Similar Products
Product Notes
The MANF / ARMET manf (Catalog #AAA2546724) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse MANF comprises 169 amino acids and has a calculated molecular mass of 19.6 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the MANF / ARMET manf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MANF / ARMET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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