ARMET/MANF Recombinant Protein | MANF recombinant protein

ARMET/MANF Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121898

• Gene Names: MANF; ARP; ARMET
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ARMET/MANF; ARMET/MANF Protein; Human; Recombinant (hFc Tag); Human MANF/ARMET Protein (Fc Tag); ARMET Protein; ARP Protein; mesencephalic astrocyte-derived neurotrophic factor; MANF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu182

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 25
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human MANF (NP_006001.4)(Met1-Leu182) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MANF recombinant protein

Background: Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF, and ARMET, is a secreted protein that belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral midbrain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus, and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggests that it has important functions both under normal and pathological conditions in many tissue types.

References

Shridhar V., et al., 1996, Oncogene 12:1931-1939. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Petrova P., et al., 2003, J. Mol. Neurosci. 20:173-188. Lindholm, P. et al., 2008, Mol Cell Neurosci. 39 (3):356-71. Airavaara, M. et al., 2010, Exp Neurol. 225 (1):104-13.

NCBI and Uniprot Product Information

• NCBI GI #: 618857509
• NCBI GeneID: 7873
• NCBI Accession #: NP_006001.4
• NCBI GenBank Nucleotide #: NM_006010.5
• UniProt Accession #: P55145; Q14CX4; Q86U67; Q96IS4
• Molecular Weight: 20,700 Da
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor; arginine-rich, mutated in early stage tumors
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: Alpha-mannosidase
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

NCBI Description

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; perinuclear region of cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: growth factor activity

Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

Disease: Pancreatic Cancer