Rat Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) ELISA Kit | MANF elisa kit

Rat Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) ELISA Kit

Cat. Num. AAA2704346

• Gene Names: Manf; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
• Reactivity: Rat
• Synonyms: Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF); Rat Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) ELISA Kit; ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein; MANF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF).; No significant cross-reactivity or interference between Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) and analogues was observed.
• Sequence Length: 179

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MANF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110625813
• NCBI GeneID: 74840
• NCBI Accession #: NP_083379.2
• NCBI GenBank Nucleotide #: NM_029103.3
• UniProt Accession #: Q9CXI5
• Molecular Weight: 20,374 Da
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: Manf
• NCBI Official Synonym Symbols: Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: Mannan endo-1,4-beta-mannosidase
• UniProt Gene Name: Manf
• UniProt Synonym Gene Names: Armet

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 F1|9 57.98 cM

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; nucleus

Molecular Function: growth factor activity; RNA binding

Biological Process: dopaminergic neuron differentiation; neuron projection development; response to unfolded protein; vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure