Typical Testing Data/Standard Curve (for reference only)
Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit | MANF clia kit
Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit
Gene Names
Manf; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
Reactivity
Human
Synonyms
MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor); Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit; MANF clia kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant MANF. No significant cross-reactivity or interference between MANF and analogues was observed.
Sequence Length
179
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MANF clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of MANF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to MANF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MANF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MANF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of MANF. You can calculate the concentration of MANF in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to MANF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MANF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MANF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of MANF. You can calculate the concentration of MANF in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,374 Da
NCBI Official Full Name
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Synonym Full Names
mesencephalic astrocyte-derived neurotrophic factor
NCBI Official Symbol
Manf
NCBI Official Synonym Symbols
Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
NCBI Protein Information
mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
Protein Family
UniProt Gene Name
Manf
UniProt Synonym Gene Names
Armet
UniProt Entry Name
MANF_MOUSE
Uniprot Description
ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular region; nucleus
Molecular Function: growth factor activity
Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein
Research Articles on MANF
Similar Products
Product Notes
The Human MANF manf (Catalog #AAA2531434) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531434 CLIA Kit recognizes Human MANF. It is sometimes possible for the material contained within the vial of "MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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