MAG / GMA / Siglec-4 Recombinant Protein | MAG / GMA recombinant protein

Mouse MAG / GMA / Siglec-4 Protein (His Tag)

Cat. Num. AAA2546617

• Gene Names: Mag; Gma; siglec-4a
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: MAG / GMA / Siglec-4; Mouse MAG / GMA / Siglec-4 Protein (His Tag); MAG; GMA; Siglec-4; MAG / GMA recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 582

• Application Notes: The recombinant mouse Mag consists of 508 amino acids and predicts a molecular mass of 56.3 kDa.
• Predicted N Terminal: Gly 20
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MAG / GMA recombinant protein

Background: The myelin-associated glycoprotein (MAG) contains five immunoglobulin-like domains and belongs to the sialic-acid-binding subgroup of the Ig superfamily. MAG is a transmembrane glycoprotein of 100kDa localized in myelin sheaths of periaxonal Schwann cell and oligodendroglial membranes where it functions in glia-axon interactions. It appears to function both as a receptor for an axonal signal that promotes the differentiation, maintenance and survival of oligodendrocytes and as a ligand for an axonal receptor that is needed for the maintence of myelinated axons. MAG contains a carbohydrate epitope shared with other glycoconjugates that is a target antigen in autoimmune peripheral neuropathy associated with IgM gammopathy and has been implicated in a dying back oligodendrogliopathy in multiple sclerosis. MAG is considered as a transmembrane protein of both CNS and PNS myelin and it strongly inhibits neurite outgrowth in both developing cerebellar and adult dosal root ganglion neurons. In contrast, MAG promotes neurite outgrowth from newborn DRG neurons. Thus, MAG may be responsible for the lack of CNS nerve regeneration and may influce both temporally and spatially regeneration in the PNS.

Description: A DNA sequence encoding the mouse Mag (NP_034888.1) (Met1-Pro516) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6754614
• NCBI GeneID: 17136
• NCBI Accession #: NP_034888.1
• NCBI GenBank Nucleotide #: NM_010758.3
• UniProt Accession #: P20917; P16880
• Molecular Weight: 64,268 Da
• NCBI Official Full Name: myelin-associated glycoprotein isoform 3
• NCBI Official Synonym Full Names: myelin-associated glycoprotein
• NCBI Official Symbol: Mag
• NCBI Official Synonym Symbols: Gma; siglec-4a
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• UniProt Gene Name: Mag

NCBI Description

This gene encodes a type I membrane protein and member of the immunoglobulin-like superfamily. It is expressed in myelinating glial cells, including oligodendrocytes of the central nervous system and Schwann cells of the peripheral nervous system. Mice lacking the encoded protein express abundant myelin, but suffer long-term axon degeneration, altered distribution of channels and adhesion molecules at nodes of Ranvier, and altered axon cytoskeletal structure. While not required for myelination, the encoded protein enhances axon-myelin stability, helps to structure nodes of Ranvier, and regulates the axon cytoskeleton. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Uniprot Description

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7 B1|7 19.26 cM

Cellular Component: cytoplasm; myelin sheath; paranode region of axon; plasma membrane

Molecular Function: protein binding; protein kinase binding; receptor binding

Biological Process: negative regulation of neuron differentiation; positive regulation of astrocyte differentiation; positive regulation of myelination