Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Derlin-3 (DERL3) Standard Curve.)
Mouse Derlin-3 (DERL3) ELISA Kit | DERL3 elisa kit
Mouse Derlin-3 (DERL3) ELISA Kit
Gene Names
Derl3; IZP6; derlin-3; 1810006I20Rik; 1810063P04Rik
Reactivity
Mouse
Synonyms
Derlin-3 (DERL3); Mouse Derlin-3 (DERL3) ELISA Kit; DERL3; C22orf14; IZP6; LLN2; MGC71803; derlin-3; DERtrin 3; der1-like protein 3; derlin 3; DERL3 elisa kit
Reactivity
Mouse
Specificity
Mouse Derlin-3 (DERL3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DERL3. No significant cross-reactivity or interference between Mouse DERL3 and analogues was observed.
Sequence Length
228
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Derlin-3 (DERL3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Derlin-3 (DERL3) Standard Curve.)
Related Product Information for DERL3 elisa kit
Description: This Mouse Derlin-3 (DERL3) ELISA Kit employs a two-site sandwich ELISA to quantitate DERL3 in samples. An antibody specific for DERL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DERL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DERL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DERL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by DERL3 belongs to the derlin family, and resides in the endoplasmic reticulum (ER). Proteins that are unfolded or misfolded in the ER must be refolded or degraded to maintain the homeostasis of the ER. This protein appears to be involved in the degradation of misfolded glycoproteins in the ER. Several alternatively spliced transcript variants encoding different isoforms have been identified for DERL3.
Background: The protein encoded by DERL3 belongs to the derlin family, and resides in the endoplasmic reticulum (ER). Proteins that are unfolded or misfolded in the ER must be refolded or degraded to maintain the homeostasis of the ER. This protein appears to be involved in the degradation of misfolded glycoproteins in the ER. Several alternatively spliced transcript variants encoding different isoforms have been identified for DERL3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
derlin-3 isoform 1
NCBI Official Synonym Full Names
Der1-like domain family, member 3
NCBI Official Symbol
Derl3
NCBI Official Synonym Symbols
IZP6; derlin-3; 1810006I20Rik; 1810063P04Rik
NCBI Protein Information
derlin-3
UniProt Protein Name
Derlin-3
Protein Family
UniProt Gene Name
Derl3
UniProt Synonym Gene Names
Der3; Izp6
Uniprot Description
DERL3: Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal glycoproteins, but not that of misfolded nonglycoproteins. May act by forming a channel that allows the retrotranslocation of misfolded glycoproteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate. Belongs to the derlin family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10|10 C1
Cellular Component: integral to endoplasmic reticulum membrane
Biological Process: ER-associated protein catabolic process; protein amino acid N-linked glycosylation via asparagine; unfolded protein response
Research Articles on DERL3
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Product Notes
The Mouse DERL3 derl3 (Catalog #AAA9717914) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717914 ELISA Kit recognizes Mouse DERL3. It is sometimes possible for the material contained within the vial of "Derlin-3 (DERL3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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