Rabbit MAG Polyclonal Antibody | anti-MAG antibody

MAG Polyclonal Antibody

Cat. Num. AAA3007073

• Gene Names: MAG; GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAG; Polyclonal Antibody; MAG Polyclonal Antibody; Myelin-associated glycoprotein; Siglec-4a; GMA; anti-MAG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAG polyclonal antibody detects endogenous levels of MAG protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 626

• Applicable Applications for anti-MAG antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MAG
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAG polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:H9C2 whole cell lysate)

Related Product Information for anti-MAG antibody

Myelin-associated glycoprotein (MAG) is a nervous system cell-surface adhesion protein that is involved in linking myelinating glial cells to neuronal axons. MAG contains a sialic acid binding site and five IgG-like domains, thus identifying MAG as a member of a subgroup of the immunoglobulin superfamily. Like myelin, MAG inhibits axonal outgrowth and contributes to the inhibitory properties of myelin. Growth inhibition by MAG has been shown to be blocked when cerebellar neurons are pre-incubated with the neurotrophins BDNF or GDNF. It is suggested that this neurotrophin priming elevates cAMP and activates PKA.

NCBI and Uniprot Product Information

• NCBI GI #: 11225258
• NCBI GeneID: 4099
• NCBI Accession #: NP_002352.1
• NCBI GenBank Nucleotide #: NP_002352.1
• UniProt Accession #: P20916
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: myelin-associated glycoprotein isoform a
• NCBI Official Synonym Full Names: myelin associated glycoprotein
• NCBI Official Symbol: MAG
• NCBI Official Synonym Symbols: GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Magainins
• UniProt Gene Name: MAG
• UniProt Synonym Gene Names: GMA
• UniProt Entry Name: MAG_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: paranode region of axon; integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; blood coagulation; cell adhesion; leukocyte migration