MAG recombinant protein

MAG Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123216

• Gene Names: Mag; Gma; siglec-4a
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MAG; MAG Protein; Mouse; Recombinant (His Tag); Mouse MAG/GMA/Siglec-4 Protein (His Tag); Gma Protein; siglec-4a Protein; myelin associated glycoprotein; MAG recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro516

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gly 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Mag (NP_034888.1)(Met1-Pro516) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MAG recombinant protein

Background: The myelin-associated glycoprotein (MAG) contains five immunoglobulin-like domains and belongs to the sialic-acid-binding subgroup of the Ig superfamily. MAG is a transmembrane glycoprotein of 100kDa localized in myelin sheaths of Periaxonal Schwann cell and oligodendroglial membranes where it functions in glia-axon interactions. It appears to function both as a receptor for an axonal signal that promotes the differentiation, maintenance, and survival of oligodendrocytes and as a ligand for an axonal receptor that is needed for the maintenance of myelinated axons. MAG contains a carbohydrate epitope shared with other glycoconjugates that is a target antigen in autoimmune peripheral neuropathy associated with IgM gammopathy and has been implicated in a dying back oligodendrogliopathy in multiple sclerosis. MAG is considered as a transmembrane protein of both CNS and PNS myelin and it strongly inhibits neurite outgrowth in both developing cerebellar and adult dorsal root ganglion neurons. In contrast, MAG promotes neurite outgrowth from newborn DRG neurons. Thus, MAG may be responsible for the lack of CNS nerve regeneration and may influence both temporally and spatially regeneration in the PNS.

References

Quarles RH. (2007) Myelin-associated glycoprotein (MAG): past, present and beyond. J Neurochem. 100(6):1431-48.Mukhopadhyay G, et al. (1994) A novel role for myelin-associated glycoprotein as an inhibitor of axonal regeneration. Neuron. 13(3): 757-67.Barton DE, et al. (1987) The myelin-associated glycoprotein gene: mapping to human chromosome 19 and mouse chromosome 7 and expression in quivering mice. Genomics. 1(2): 107-12.

NCBI and Uniprot Product Information

• NCBI GI #: 6754614
• NCBI GeneID: 17136
• NCBI Accession #: NP_034888.1
• NCBI GenBank Nucleotide #: NM_010758.2
• UniProt Accession #: P20917; P16880
• Molecular Weight: 69,260 Da
• NCBI Official Full Name: myelin-associated glycoprotein
• NCBI Official Synonym Full Names: myelin-associated glycoprotein
• NCBI Official Symbol: Mag
• NCBI Official Synonym Symbols: Gma; siglec-4a
• NCBI Protein Information: myelin-associated glycoprotein
• UniProt Protein Name: Myelin-associated glycoprotein
• Protein Family: Magainins
• UniProt Gene Name: Mag
• UniProt Entry Name: MAG_MOUSE