DR6 / TNFRSF21 recombinant protein

Recombinant Human DR6 / TNFRSF21 Protein

Cat. Num. AAA2546451

• Gene Names: TNFRSF21; DR6; CD358; BM-018
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: DR6 / TNFRSF21; Recombinant Human DR6 / TNFRSF21 Protein; BM-018; DR6; MGC31965; TNFRSF21; DR6 / TNFRSF21 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 655

• Application Notes: The recombinant human DR6 consists of 315 amino acids and predicts a molecular mass of 34.2 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhDR6 is approximately 55-60 kDa due to the glycosylation.
• Predicted N Terminal: Gln 42
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DR6 / TNFRSF21 recombinant protein

Background: TNFRSF21 (death receptor-6, DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain, and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production, suggested that DR6 serves as an important regulatory molecule in T-helper cell activation, and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs, heart, brain and pancreas. Some tumor cells overexpress DR6, typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion, which is subject to future investigations.

Description: The mature form of human DR6 (NP_055267.1) extracellular domain (Met 1-Leu 350) with five amino acids (DDDDK) at the C-terminus was expressed and purified.

NCBI and Uniprot Product Information

• NCBI GI #: 7657039
• NCBI GeneID: 27242
• NCBI Accession #: NP_055267.1
• NCBI GenBank Nucleotide #: NM_014452.4
• UniProt Accession #: O75509; Q0D2P5; Q96D86; B2RDI9
• Molecular Weight: 71,845 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: TNF receptor superfamily member 21
• NCBI Official Symbol: TNFRSF21
• NCBI Official Synonym Symbols: DR6; CD358; BM-018
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• UniProt Gene Name: TNFRSF21
• UniProt Synonym Gene Names: DR6

NCBI Description

This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation. [provided by RefSeq, Jul 2013]

Uniprot Description

DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.

Protein type: Apoptosis; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: integral to plasma membrane; intrinsic to plasma membrane; plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity

Biological Process: adaptive immune response; apoptosis; B cell apoptosis; humoral immune response; immune response; inflammatory response; myelination; negative regulation of B cell proliferation; negative regulation of myelination; negative regulation of T cell proliferation; neuron apoptosis; regulation of apoptosis; regulation of cell proliferation; regulation of oligodendrocyte differentiation; response to lipopolysaccharide; T cell receptor signaling pathway