Mouse NGAL ELISA Kit | LCN2 elisa kit

Mouse NGAL ELISA Kit

Cat. Num. AAA824498

• Gene Names: Lcn2; 24p3; Sip24; AW212229
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: NGAL; Mouse NGAL ELISA Kit; HNL; Neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25; LCN2 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse NGAL ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse NGAL proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 200

• Applicable Applications for LCN2 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Urine
• Assay Type: Quantitative Sandwich
• Sensitivity: <8 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCN2 elisa kit

Principle of the Assay: The MyBioSource' Mouse NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse NGAL in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Mouse NGAL coated on a 96-well plate. Standards and samples are pipetted into the wells and NGAL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse NGAL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGAL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. The LCN2 gene is located at 9q34 and contains 7 exons. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 34328049
• NCBI GeneID: 16819
• NCBI Accession #: NP_032517.1
• NCBI GenBank Nucleotide #: NM_008491.1
• UniProt Accession #: P11672; Q3UE34
• Molecular Weight: 22,875 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: Lcn2
• NCBI Official Synonym Symbols: 24p3; Sip24; AW212229
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; siderocalin LCN2; SV-40-induced 24P3 protein; secreted inducible protein 24
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: Lcn2
• UniProt Synonym Gene Names: NGAL
• UniProt Entry Name: NGAL_MOUSE

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: protein binding; protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; iron ion homeostasis; immune system process; transport; apoptosis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide