Related Product Information for LBP recombinant protein
Background: Lipopolysaccharide binding protein (LBP) is a glycoprotein that is synthesized principally by hepatocytes. LBP is a trace plasma protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPSs). LBP binds directly to the outer membrane of Gram-negative bacteria and to purified aggregates of extracted endotoxin, and catalyses the delivery of endotoxin to membrane (mCD14, GPI-Linked) and soluble (sCD14) forms of CD14, thereby markedly increasing host cell sensitivity to endotoxin. LBP efficiently catalyses the transfer of individual molecules of endotoxin to (s)CD14 only when LBP-endotoxin aggregates are formed in the presence of albumin. In the presence of EDTA, LBP binding promotes further disaggregation of endotoxin. LBP binding does not have such drastic effects under more physiological conditions, but may still induce more subtle topological rearrangements of endotoxin.
Description: A DNA sequence encoding the human LBP (NP_004130.2) extracellular domain (Met 1-Val 481) was fused with the a polyhistidine tag at the C-terminus.
NCBI Description
The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]
Uniprot Description
LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20q11.23
Cellular Component: cell surface; extracellular region; extracellular space
Molecular Function: lipopolysaccharide binding; protein binding; receptor binding
Biological Process: acute-phase response; cellular defense response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of tumor necrosis factor production; opsonization; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor production; response to lipopolysaccharide; toll-like receptor 4 signaling pathway
Product Notes
The LBP lbp (Catalog #
AAA2546202) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human LBP consists of 467 amino acids and has a predicted molecular mass of 52.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLBP is approximately 62 kDa due to glycosylation. Researchers should empirically determine the suitability of the LBP lbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"LBP / Lipopolysaccharide binding, Recombinant Protein" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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