Mouse anti-Human ATF6 Monoclonal Antibody | anti-ATF6 antibody

Mouse Monoclonal [clone 3D5] (IgG2a,k) to Human ATF6

Cat. Num. AAA245735

• Gene Names: ATF6; ATF6A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ATF6; Monoclonal Antibody; Mouse Monoclonal [clone 3D5] (IgG2a; k) to Human ATF6; Anti-ATF6 Antibody (clone 3D5) IHC-plus; ATF6-alpha; ATF6A; Human ATF6; anti-ATF6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3D5
• Specificity: Human ATF6
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.36 mg/ml (varies by lot)
• Sequence Length: 670

• Applicable Applications for anti-ATF6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot with the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: ATF6 (AAH14969, 91 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ATF6 antibody was raised against aTF6 (AAH14969, 91 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATF6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-ATF6 antibody

Activating Transcription Factor 6 (ATF6) is a type II transmembrane glycoprotein located on the ER membrane. In response to ER stress ATF6 is cleaved and the N-terminus including the leucine zipper (which faces the cytosol) moves to the nucleus. Once in the nucleus ATF6 binds to the ER stress response elements in ER stress genes. Site-1 and site-2 protease enzymes that process sterol regulatory binding proteins in response to cholesterol derivation cleave ATF6.

NCBI and Uniprot Product Information

• NCBI GI #: 56786157
• NCBI GeneID: 22926
• NCBI Accession #: NP_031374.2
• NCBI GenBank Nucleotide #: NM_007348.3
• UniProt Accession #: P18850; O15139; Q5VW62; Q6IPB5; Q9UEC9
• Molecular Weight: 74,585 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-6 alpha
• NCBI Official Synonym Full Names: activating transcription factor 6
• NCBI Official Symbol: ATF6
• NCBI Official Synonym Symbols: ATF6A
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 alpha
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF6
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
• UniProt Entry Name: ATF6A_HUMAN

NCBI Description

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Uniprot Description

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; transcription, DNA-dependent; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction

Disease: Achromatopsia 7