LBP recombinant protein

LBP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120333

• Gene Names: LBP; BPIFD2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LBP; LBP Protein; Human; Recombinant (His Tag); Human LBP/Lipopolysaccharide binding Protein (His Tag); BPIFD2 Protein; lipopolysaccharide binding protein; LBP recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM NaCl, 0.05mM EDTA, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val481

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LBP (NP_004130.2) extracellular domain (Met 1-Val 481) was fused with the a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LBP recombinant protein

Background: Lipopolysaccharide binding protein (LBP) is a glycoprotein that is synthesized principally by hepatocytes. LBP is a trace plasma protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPSs). LBP binds directly to the outer membrane of Gram-negative bacteria and purified aggregates of extracted endotoxin and catalyzes the delivery of endotoxin to the membrane (mCD14, GPI-Linked) and soluble (sCD14) forms of CD14, thereby markedly increasing host cell sensitivity to endotoxin. LBP efficiently catalyzes the transfer of individual molecules of endotoxin to (s)CD14 only when LBP-endotoxin aggregates are formed in the presence of albumin. In the presence of EDTA, LBP binding promotes further disaggregation of endotoxin. LBP binding does not have such drastic effects under more physiological conditions, but may still induce more subtle topological rearrangements of endotoxin.

Product Categories/Family for LBP recombinant protein

NF-kB (NFkB) Pathway

References

J. Weiss, 2003, Biochemical Society Transactions: Volume 31, part 4: 785-790. RR Schumann, et al., Science, 1990, Vol 249, Issue 4975: 1429-143. Carsten J. Kirschning, et al., 1997, Genomics, Volume 46, Issue 3: 416-25.

NCBI and Uniprot Product Information

• NCBI GI #: 31652249
• NCBI GeneID: 3929
• NCBI Accession #: NP_004130.2
• NCBI GenBank Nucleotide #: NM_004139.3
• UniProt Accession #: P18428; O43438; Q92672; Q9H403; Q9UD66; B2R938
• Molecular Weight: 53,384 Da
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein
• NCBI Official Symbol: LBP
• NCBI Official Synonym Symbols: BPIFD2
• NCBI Protein Information: lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2
• UniProt Protein Name: Lipopolysaccharide-binding protein
• Protein Family: Lipopolysaccharide-binding protein
• UniProt Gene Name: LBP
• UniProt Synonym Gene Names: LBP
• UniProt Entry Name: LBP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

Uniprot Description

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: protein binding; lipopolysaccharide binding; receptor binding

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; acute-phase response; toll-like receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; cellular defense response; innate immune response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation