Human LBP PicoKine ELISA Kit | LBP elisa kit

Human LBP PicoKine ELISA Kit

Cat. Num. AAA177608

• Gene Names: LBP; BPIFD2
• Reactivity: Human
• Synonyms: LBP PicoKine; Human LBP PicoKine ELISA Kit; Lipopolysaccharide-binding protein; BPI fold containing family D; member 2; Bpifd2; LBP; LBP_HUMAN; LBP1; Lipopolysaccharide binding protein; LPS binding protein; Ly88; MGC22233; OTTHUMP00000030965; RP23-407H16.4; lipopolysaccharide binding protein; LBP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LBP
• Sequence Length: 481

• Assay Range: 1.56ng/ml-100ng/ml
• Sensitivity: <50pg/ml
• Sample: For quantitative detection of human LBP in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO,A26-V481
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 1.25ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human LBP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human LBP standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|15.3|38|85|20|42|93; Standard deviation|0.66|1.9|4.76|1.48|2.77|6.7; CV(%)|4.3|5|5.6|7.4|6.6|7.2
• Sample Data: Concentration(ng/ml)|0|1.56|3.12|6.25|12.5|25|50|100; O.D.|0.103|0.217|0.289|0.463|0.731|1.175|1.903|2.402
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human LBP PicoKine ELISA Kit standard curve )

Related Product Information for LBP elisa kit

Principle of the assay: Human LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LBP has been precoated onto 96-well plates. Standards(NSO, A26-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LBP amount of sample captured in plate.

Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

NCBI and Uniprot Product Information

• NCBI GI #: 31652249
• NCBI GeneID: 3929
• NCBI Accession #: NP_004130.2
• NCBI GenBank Nucleotide #: NM_004139.4
• UniProt Accession #: P18428; O43438; Q92672; Q9H403; Q9UD66; B2R938
• Molecular Weight: 53,384 Da
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein
• NCBI Official Symbol: LBP
• NCBI Official Synonym Symbols: BPIFD2
• NCBI Protein Information: lipopolysaccharide-binding protein
• UniProt Protein Name: Lipopolysaccharide-binding protein
• Protein Family: Lipopolysaccharide-binding protein
• UniProt Gene Name: LBP
• UniProt Synonym Gene Names: LBP
• UniProt Entry Name: LBP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

Uniprot Description

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cell surface; extracellular region; extracellular space; membrane

Molecular Function: lipopolysaccharide binding; protein binding; receptor binding

Biological Process: acute-phase response; cellular defense response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; leukocyte chemotaxis during inflammatory response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; liver development; macrophage activation during immune response; negative regulation of tumor necrosis factor production; opsonization; positive regulation of chemokine production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of phagocytosis, engulfment; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; response to lipopolysaccharide; toll-like receptor 4 signaling pathway