Western Blot (WB)
(Top Gel: Recombinant Human Annexin A1 (10 ng) and cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at 1:3000. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant Human Annexin A1; Lane 2: HeLa cell lysate; Lane 3: A549 cell lysate; Lane 4: A431 cell lysate. Bottom Gel: HeLa cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at either 1:10,000 (Lane 1) 1:20,000 (Lane 2).)
Mouse Annexin 1 Monoclonal Antibody | anti-ANNAT1 antibody
Mouse Monoclonal Annexin 1 antibody
Western Blot (WB)
(Top Gel: Recombinant Human Annexin A1 (10 ng) and cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at 1:3000. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant Human Annexin A1; Lane 2: HeLa cell lysate; Lane 3: A549 cell lysate; Lane 4: A431 cell lysate. Bottom Gel: HeLa cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at either 1:10,000 (Lane 1) 1:20,000 (Lane 2).)
Western Blot (WB)
(Top Gel: Recombinant Human Annexin A1 (10 ng) and cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at 1:3000. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Recombinant Human Annexin A1; Lane 2: HeLa cell lysate; Lane 3: A549 cell lysate; Lane 4: A431 cell lysate. Bottom Gel: HeLa cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Annexin A1 antibody at either 1:10,000 (Lane 1) 1:20,000 (Lane 2).)
NCBI and Uniprot Product Information
NCBI Description
Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, beta-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion. It is a Ca 2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca 2+ in plants.
Uniprot Description
Has a peroxidase activity. May act in counteracting oxidative stress. May also mediate regulated, targeted secretion of Golgi-derived vesicles during seedling development.
Research Articles on ANNAT1
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Product Notes
The ANNAT1 ann1 (Catalog #AAA5305154) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Annexin 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:3000-1:20000. Researchers should empirically determine the suitability of the ANNAT1 ann1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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