KMT2C recombinant protein

Recombinant KMT2C (MLL3) complex

Cat. Num. AAA388189

• Gene Names: KMT2C; HALR; MLL3
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: KMT2C; Recombinant KMT2C (MLL3) complex; HALR; KMT2C recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Full length Recombinant KMT2C (MLL3) complex expressed in E Coli cells and supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

• Protein Species: Human
• Tag: GST-Tag, His-Tag
• Notes: Recombinant KMT2C (MLL3) complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions:50mM TrisCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM SAM, 10nm Recombinant KMT2C (MLL3) complex, 3.3uM H3 (1-21) peptide at 2 hours at room temperature.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant KMT2C (MLL3) complex protein gel. Recombinant KMT2C (MLL3) complex run on an SDS-PAGE gel and stained with Coomassie Blue.)

Testing Data

(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2C (MLL3) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)

Testing Data

(Recombinant KMT2C (MLL3) complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM Recombinant KMT2C (MLL3) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~165 turnovers/ enzyme molecule)

Related Product Information for KMT2C recombinant protein

Short Description: Recombinant KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30) was generated using a peptide corresponding to amino acids 4740-4911 (accession number NP_733751.2) of the KMT2C protein that was expressed in E Coli. Recombinant KMT2C (MLL3) complex contains an N-terminal GST-Tag with a molecular weight of 46 kDa; human WDR5 (accession number NM_017588) that contains an N-terminal His-Tag with a molecular weight of 35 kDa; human ASH2L (accession number NM_001105214) that contains an N-terminal His-Tag with a molecular weight of 61 kDa; human RBBP5 (accession number NM_005057) that contains an N-terminal His-Tag with a molecular weight of 60 kDa; and human DPY30 (accession number NM_032574) that contains an N-terminal His-Tag with a molecular weight of 12 kDa. All Recombinant MLL3/WDR5/ASH2L/RBBP5/DPY30 complex proteins were individually expressed in an E Coli expression system. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: The KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The KMT2C (also known as MLL3) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer.

Product Categories/Family for KMT2C recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT)

NCBI and Uniprot Product Information

• NCBI GI #: 91718902
• NCBI GeneID: 58508
• NCBI Accession #: NP_733751.2
• NCBI GenBank Nucleotide #: NM_017588
• UniProt Accession #: Q8NEZ4; Q8NC02; Q8NDF6; Q9H9P4; Q9NR13; Q9P222; Q9UDR7
• Molecular Weight: 541,370 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase 2C
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2C
• NCBI Official Symbol: KMT2C
• NCBI Official Synonym Symbols: HALR; MLL3
• NCBI Protein Information: histone-lysine N-methyltransferase 2C; ALR-like protein; homologous to ALR protein; histone-lysine N-methyltransferase MLL3; myeloid/lymphoid or mixed-lineage leukemia protein 3; histone-lysine N-methyltransferase, H3 lysine-4 specific
• UniProt Protein Name: Histone-lysine N-methyltransferase 2C
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: KMT2C
• UniProt Synonym Gene Names: HALR; KIAA1506; MLL3; Lysine N-methyltransferase 2C

Uniprot Description

MLL3: a protein methyltransferase that methylates K4 of histone H3. H3K4 methylation represents a specific tag for epigenetic transcriptional activation. Found in a critical region of chromosome 7, which is commonly deleted in malignant myeloid disorders. Partial duplication of the MLL3 gene are found in the juxtacentromeric region of chromosomes 1, 2, 13 and 21. Central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional coactivation. MLL3 may be a catalytic subunit of this complex. Contributes to genome-scale circadian transcription. May be involved in leukemogenesis and developmental disorder. Highly expressed in testis and ovary, followed by brain and liver. Also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. Not detected in skeletal muscle and fetal liver. Belongs to the protein lysine methyltransferase family. TRX/MLL subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: histone methyltransferase complex; nucleoplasm; nucleus

Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; RNA binding