SDS-Page
(Recombinant KMT2B (MLL4) complex gel KMT2B (MLL4) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
KMT2B recombinant protein
Recombinant KMT2B (MLL4) complex
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KMT2B (MLL4) complex gel KMT2B (MLL4) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant KMT2B (MLL4) complex gel KMT2B (MLL4) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(Recombinant KMT2B (MLL4) complex HTRF activity assay 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2B (MLL4) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2B (MLL4) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Testing Data
(Recombinant KMT2B (MLL4) complex HTRF activity assay 3.3 uM H3K4me0 (1-21aa) peptide was incubated with Recombinant KMT2B (MLL4) complex in reaction buffer for 3 hour at room temperature. Recombinant KMT2B (MLL4) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
Testing Data
(Recombinant KMT2B (MLL4) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 20 nM Recombinant KMT2B (MLL4) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: 50 turnovers/ enzyme molecule)
Testing Data
(Recombinant KMT2B (MLL4) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 20 nM Recombinant KMT2B (MLL4) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: 50 turnovers/ enzyme molecule)
Background: KMT2B (MLL4)(Myeloid/lymphoid or mixed-lineage leukemia 4) is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL4) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in b-globin locus transcription regulation by being recruited by Nfe2. KMT2B (MLL4) is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]
Uniprot Description
MLL2: may be a transcriptional regulator. Contains 3 A.T hook DNA-binding domains. Two alternatively spliced isoforms have been described.
Protein type: Methyltransferase; EC 2.1.1.43; Transcription factor; Methyltransferase, protein lysine; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription factor activity
Biological Process: ovulation; establishment and/or maintenance of chromatin architecture; ovarian follicle development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone H3-K4 methylation; oocyte differentiation; regulation of histone H3-K4 methylation; gene silencing
Research Articles on KMT2B
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Product Notes
The KMT2B kmt2b (Catalog #AAA388209) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KMT2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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