Mouse Progonadoliberin-1 ELISA Kit | Gnrh1 elisa kit

Mouse Progonadoliberin-1 ELISA Kit

Cat. Num. AAA760179

• Gene Names: GNRH1; GRH; GNRH; LHRH; LNRH
• Reactivity: Mouse
• Synonyms: Progonadoliberin-1; Mouse Progonadoliberin-1 ELISA Kit; Gnrh1/Progonadoliberin-1/GNRH/GRHLHRH/Progonadoliberin I; Gnrh1 elisa kit

• Reactivity: Mouse
• Sequence Length: 92

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Gnrh1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Gnrh1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Gnrh1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Gnrh1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Gnrh1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 133908612
• NCBI GeneID: 2796
• NCBI Accession #: NP_001076580.1
• NCBI GenBank Nucleotide #: NM_001083111.1
• Molecular Weight: 10,380 Da
• NCBI Official Full Name: progonadoliberin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: gonadotropin releasing hormone 1
• NCBI Official Symbol: GNRH1
• NCBI Official Synonym Symbols: GRH; GNRH; LHRH; LNRH
• NCBI Protein Information: progonadoliberin-1
• UniProt Protein Name: Progonadoliberin-1
• Protein Family: Progonadoliberin
• UniProt Gene Name: GNRH1
• UniProt Synonym Gene Names: GNRH; GRH; LHRH; GnRH-I; LH-RH I
• UniProt Entry Name: GON1_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism. [provided by RefSeq, Nov 2015]

Uniprot Description

GNRH1: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Belongs to the GnRH family.

Protein type: Hormone; Secreted; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p21-p11.2

Cellular Component: dendrite; extracellular region; extracellular space; Golgi-associated vesicle; mitochondrion; nerve terminal; perikaryon

Molecular Function: hormone activity; luteinizing hormone-releasing factor activity

Biological Process: aging; cell-cell signaling; female pregnancy; male sex determination; multicellular organismal development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of immature T cell proliferation; regulation of gene expression; response to corticosteroid stimulus; response to ethanol; response to lipopolysaccharide; response to testosterone stimulus; signal transduction

Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia