SDS-Page
(Recombinant DOT1L (1-416) protein gel. Recombinant DOT1L (1-416), protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72 kDa Purity: > 80%)
DOT1L recombinant protein
Recombinant DOT1L (1-416) protein
Gene Names
DOT1L; DOT1; KMT4
Synonyms
DOT1L; Recombinant DOT1L (1-416) protein; DOT1; KMT4; DOT1L recombinant protein
Host
E Coli
Form/Format
Recombinant DOT1L (1-416) protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 2ug recombinant nucleosomes were incubated with DOT1L in reaction buffer including 50mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 50uM SAM for 3 hours at room temperature. Western blot was used for activity detection. Western Blot was used to detect the generation of reaction products (Anti-H3K79me1, 1:000 dilution). DOT1L only was used as negative control.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant DOT1L (1-416) protein gel. Recombinant DOT1L (1-416), protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72 kDa Purity: > 80%)
SDS-Page
(Recombinant DOT1L (1-416) protein gel. Recombinant DOT1L (1-416), protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 72 kDa Purity: > 80%)
Western Blot (WB)
(Recombinant DOT1L (1-416) protein activity. 2 ug recombinant nucleosomes were incubated with DOT1L protein for 3 hrs at RT and reaction products detected by Western Blot (Anti-H3K79me1) with DOT1L protein alone as a negative control. The activity of DOT1L with nucleosomes as substrates is greater than with histone octamer substrates.)
Western Blot (WB)
(Recombinant DOT1L (1-416) protein activity. 2 ug recombinant nucleosomes were incubated with DOT1L protein for 3 hrs at RT and reaction products detected by Western Blot (Anti-H3K79me1) with DOT1L protein alone as a negative control. The activity of DOT1L with nucleosomes as substrates is greater than with histone octamer substrates.)
Related Product Information for DOT1L recombinant protein
Short Description: Recombinant DOT1L (1-416) protein that includes amino acids 1-416 that contains the SET domain sequence of the human DOT1L protein (accession number NP_115871.1) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 72 kDa.
Background: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.
Background: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.
Product Categories/Family for DOT1L recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
184853 MW
NCBI Official Full Name
histone-lysine N-methyltransferase, H3 lysine-79 specific
NCBI Official Synonym Full Names
DOT1 like histone lysine methyltransferase
NCBI Official Symbol
DOT1L
NCBI Official Synonym Symbols
DOT1; KMT4
NCBI Protein Information
histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Protein Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Gene Name
DOT1L
UniProt Synonym Gene Names
KIAA1814; KMT4; H3-K79-HMTase
NCBI Description
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Uniprot Description
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Research Articles on DOT1L
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Product Notes
The DOT1L dot1l (Catalog #AAA388185) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DOT1L, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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