Rabbit anti-Human JARID1A Polyclonal Antibody | anti-KDM5A antibody

JARID1A, CT (KDM5A,JARID1A)

Cat. Num. AAA6003164

• Gene Names: KDM5A; RBP2; RBBP2; RBBP-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: JARID1A; Polyclonal Antibody; CT (KDM5A; JARID1A); Anti -JARID1A; anti-KDM5A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-KDM5A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: JARID1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1608-1641 amino acids from the C-terminal region of human JARID1A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(JARID1A Antibody (C-term) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the JARID1A antibody detected the JARID1A protein (arrow).)

Immunohistochemistry (IHC)

(JARID1A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JARID1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KDM5A antibody

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein.

Product Categories/Family for anti-KDM5A antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 83406060
• NCBI GeneID: 5927
• UniProt Accession #: P29375; Q4LE72; Q86XZ1; A8MV76
• Molecular Weight: 192,095 Da
• NCBI Official Full Name: JARID1A protein
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 5A
• NCBI Official Symbol: KDM5A
• NCBI Official Synonym Symbols: RBP2; RBBP2; RBBP-2
• NCBI Protein Information: lysine-specific demethylase 5A; histone demethylase JARID1A; retinoblastoma binding protein 2; jumonji/ARID domain-containing protein 1A; Jumonji, AT rich interactive domain 1A (RBP2-like)
• UniProt Protein Name: Lysine-specific demethylase 5A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM5A
• UniProt Synonym Gene Names: JARID1A; RBBP2; RBP2; RBBP-2
• UniProt Entry Name: KDM5A_HUMAN

NCBI Description

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]

Uniprot Description

JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.11.-; Nucleolus; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; multicellular organismal development; positive regulation of transcription, DNA-dependent; chromatin modification; circadian regulation of gene expression