IZUMO1 recombinant protein

Recombinant Human IZUMO1 Protein (His tag)

Cat. Num. AAA2545292

• Gene Names: IZUMO1; OBF; IZUMO
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: IZUMO1; Recombinant Human IZUMO1 Protein (His tag); IZUMO; IZUMO1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween-80.
• Sequence: Met 1-Arg292

• Application Notes: The recombinant human IZUMO1 consists of 282 amino acids and predicts a molecular mass of 32.1 KDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water(400 ul) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for IZUMO1 recombinant protein

Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Izumo1 is essential for sperm-egg plasma membrane binding and fusion.

NCBI and Uniprot Product Information

• NCBI GI #: 22902472
• NCBI GeneID: 284359
• Molecular Weight: Mol_Mass: 32.1 kDa; AP_Mol_Mass: 43 kDa
• NCBI Official Full Name: Izumo sperm-egg fusion 1
• NCBI Official Synonym Full Names: izumo sperm-egg fusion 1
• NCBI Official Symbol: IZUMO1
• NCBI Official Synonym Symbols: OBF; IZUMO
• NCBI Protein Information: izumo sperm-egg fusion protein 1
• UniProt Protein Name: Izumo sperm-egg fusion protein 1
• Protein Family: Izumo sperm-egg fusion protein
• UniProt Gene Name: IZUMO1
• UniProt Synonym Gene Names: OBF

NCBI Description

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).[supplied by OMIM, Mar 2008]

Uniprot Description

IZUMO1: Involved in the fertilization process. May be involved in the fusion of the sperm with the egg. Belongs to the Izumo family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor binding

Biological Process: cell adhesion; fusion of sperm to egg plasma membrane; heterotypic cell-cell adhesion; single fertilization; sperm-egg recognition