IZUMO4 recombinant protein

IZUMO4 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121355

• Gene Names: IZUMO4; C19orf36; IMAGE:4215339
• Purity: >96% as determined by SDS-PAGE
• Synonyms: IZUMO4; IZUMO4 Protein; Human; Recombinant (hFc Tag); Human IZUMO4/C19orf36 Protein (Fc Tag); C19orf36 Protein; IMAGE:4215339 Protein; UNQ831/PRO1758 Protein; IZUMO family member 4; IZUMO4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His214

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 18
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human C19orf36 (Q1ZYL8)(Met1-His214) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for IZUMO4 recombinant protein

Background: Izumo is a sperm membrane protein that plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain the Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain, or both of Izumo 1 are required for the formation of multimers of a higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes, and Izumo 3 and 4 forming a single larger complex. Co-immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex.

References

Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics. 89(3):307-15.Ellerman DA, et al. (2009) Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm. Mol Reprod Dev. 76(12):1188-99.

NCBI and Uniprot Product Information

• NCBI GI #: 89903025
• NCBI GeneID: 113177
• NCBI Accession #: NP_001026905.2
• NCBI GenBank Nucleotide #: NM_001031735.2
• UniProt Accession #: Q1ZYL8; Q6UXA2; Q96FT6; Q96L02; A7RA93; A7RA94
• Molecular Weight: 26,510 Da
• NCBI Official Full Name: izumo sperm-egg fusion protein 4 isoform 1
• NCBI Official Synonym Full Names: IZUMO family member 4
• NCBI Official Symbol: IZUMO4
• NCBI Official Synonym Symbols: C19orf36; IMAGE:4215339
• NCBI Protein Information: izumo sperm-egg fusion protein 4; sperm 22 kDa protein c113; putative SAMP14 binding protein variant 1; putative SAMP14 binding protein variant 2
• UniProt Protein Name: Izumo sperm-egg fusion protein 4
• Protein Family: Izumo sperm-egg fusion protein
• UniProt Gene Name: IZUMO4
• UniProt Synonym Gene Names: C19orf36; UNQ831/PRO1758
• UniProt Entry Name: IZUM4_HUMAN