Mouse insulin-like 3 (Leydig cell) ELISA Kit | INSL3 elisa kit

Mouse Insulin-like 3, INSL3 ELISA Kit

Cat. Num. AAA924129

• Gene Names: Insl3; Rlf; Rlnl
• Reactivity: Mouse
• Synonyms: insulin-like 3 (Leydig cell); Mouse Insulin-like 3; INSL3 ELISA Kit; Mouse Insulin-like 3 (INSL3) ELISA kit; MGC119818; MGC119819; RLF; RLNL; insulin-like 3; leydig insulin -like hormone; leydig insulin -like peptide; relaxin-like factor b; INSL3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse INSL3. No significant cross-reactivity or interference between Mouse INSL3 and analogues was observed.
• Sequence Length: 122

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for INSL3 elisa kit

For Sample For the quantitative determination of mouse insulin-like 3 (INSL3) concentrations in serum, plasma, tissue homogenates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INSL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 17380363
• NCBI GeneID: 16336
• UniProt Accession #: O09107; P97744
• Molecular Weight: 13,586 Da
• NCBI Official Full Name: Insulin-like 3
• NCBI Official Synonym Full Names: insulin-like 3
• NCBI Official Symbol: Insl3
• NCBI Official Synonym Symbols: Rlf; Rlnl
• NCBI Protein Information: insulin-like 3; Ley I-L; ley-I-L; relaxin like factor; relaxin-like factor; leydig insulin-like peptide
• UniProt Protein Name: Insulin-like 3
• Protein Family: Insulin
• UniProt Gene Name: Insl3
• UniProt Synonym Gene Names: Rlf; Ley-I-L
• UniProt Entry Name: INSL3_MOUSE

Uniprot Description

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: protease binding; G-protein-coupled receptor binding; hormone activity

Biological Process: negative regulation of cell proliferation; in utero embryonic development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; male gonad development; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis