Human TXK ELISA Kit | TXK elisa kit

Human TXK (Protein tyrosine kinase 4) ELISA Kit

Cat. Num. AAA7607004

• Gene Names: TXK; RLK; TKL; BTKL; PTK4; PSCTK5
• Reactivity: Human
• Synonyms: TXK; Human TXK (Protein tyrosine kinase 4) ELISA Kit; TXK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TXK. No significant cross-reactivity or interference between TXK and analogues was observed.
• Sequence Length: 527

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TXK were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TXK were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TXK elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TXK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TXK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TXK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TXK can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148596974
• NCBI GeneID: 7294
• NCBI Accession #: NP_003319.2
• NCBI GenBank Nucleotide #: NM_003328.2
• UniProt Accession #: P42681; Q14220
• Molecular Weight: 61,258 Da
• NCBI Official Full Name: tyrosine-protein kinase TXK
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: TXK
• NCBI Official Synonym Symbols: RLK; TKL; BTKL; PTK4; PSCTK5
• NCBI Protein Information: tyrosine-protein kinase TXK
• UniProt Protein Name: Tyrosine-protein kinase TXK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: TXK
• UniProt Synonym Gene Names: PTK4; RLK

Uniprot Description

Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.