Mouse Insulin-like 3 ELISA Kit | Insl3 elisa kit

Mouse Insulin-like 3 ELISA Kit

Cat. Num. AAA289772

• Gene Names: Insl3; Rlf; Rlnl
• Reactivity: Mouse
• Synonyms: Insulin-like 3; Mouse Insulin-like 3 ELISA Kit; Leydig insulin-like peptide; Ley-I-L; Relaxin-like factor; Insl3; Rlf; Insl3 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Insulin-like 3
• Sequence Length: 122

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.081 ng/mL
• Intra-assay Precision: <=8.1%
• Inter-assay Precision: <=10.5%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Insl3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17380363
• NCBI GeneID: 16336
• UniProt Accession #: O09107; P97744
• Molecular Weight: 13,586 Da
• NCBI Official Full Name: Insulin-like 3
• NCBI Official Synonym Full Names: insulin-like 3
• NCBI Official Symbol: Insl3
• NCBI Official Synonym Symbols: Rlf; Rlnl
• NCBI Protein Information: insulin-like 3
• UniProt Protein Name: Insulin-like 3
• Protein Family: Insulin
• UniProt Gene Name: Insl3
• UniProt Synonym Gene Names: Rlf; Ley-I-L
• UniProt Entry Name: INSL3_MOUSE

Uniprot Description

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: G-protein-coupled receptor binding; hormone activity; protease binding

Biological Process: G-protein signaling, adenylate cyclase inhibiting pathway; in utero embryonic development; male gonad development; negative regulation of apoptosis; negative regulation of cell proliferation; oocyte maturation; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation