Mouse insulin-like 5 ELISA Kit | INSL5 elisa kit

Mouse Insulin-like peptide INSL5, INSL5 ELISA Kit

Cat. Num. AAA706319

• Gene Names: Insl5; RIF2
• Reactivity: Mouse
• Synonyms: insulin-like 5; Mouse Insulin-like peptide INSL5; INSL5 ELISA Kit; Mouse Insulin-like peptide INSL5 (INSL5) ELISA kit; MGC126695; MGC126697; PRO182; UNQ156; insulin-like peptide 5; INSL5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse INSL5. No significant cross-reactivity or interference between Mouse INSL5 and analogues was observed.
• Sequence Length: 145

• Samples: Serum, plasma, cell culture supernates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INSL5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INSL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 594591545
• NCBI GeneID: 23919
• NCBI Accession #: NP_001277577.1
• NCBI GenBank Nucleotide #: NM_001290648.1
• UniProt Accession #: Q9WUG6
• Molecular Weight: 15,524 Da
• NCBI Official Full Name: insulin-like peptide INSL5 isoform 1
• NCBI Official Synonym Full Names: insulin-like 5
• NCBI Official Symbol: Insl5
• NCBI Official Synonym Symbols: RIF2
• NCBI Protein Information: insulin-like peptide INSL5; relaxin/insulin-like protein; relaxin/insulin-like factor 2
• UniProt Protein Name: Insulin-like peptide INSL5
• Protein Family: Insulin-like peptide
• UniProt Gene Name: Insl5
• UniProt Synonym Gene Names: Rif; Rif2; Zins3; Insulin-like peptide 5
• UniProt Entry Name: INSL5_MOUSE

Uniprot Description

Function: May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor