Human Neprilysin (NEP) ELISA Kit | NEP elisa kit

Human Neprilysin, NEP ELISA Kit

Cat. Num. AAA702893

• Reactivity: Human
• Synonyms: Neprilysin (NEP); Human Neprilysin; NEP ELISA Kit; Human Neprilysin (NEP) ELISA Kit; NEP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NEP. No significant cross-reactivity or interference between Human NEP and analogues was observed.
• Sequence Length: 121

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: 19.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 71655461
• UniProt Accession #: Q0R5M8
• Molecular Weight: 14,365 Da
• NCBI Official Full Name: NEP
• UniProt Protein Name: NEP
• Protein Family: Halolysin-like extracellular serine protease
• UniProt Entry Name: Q0R5M8_9INFA

Uniprot Description

Function: Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced SAAS annotationsSAAS:SAAS000968