Bovine Insulin-like 3 (INSL3) ELISA Kit | INSL3 elisa kit

Bovine Insulin-like 3 (INSL3) ELISA Kit

Cat. Num. AAA7241234

• Gene Names: INSL3; RLF; RLNL; ley-I-L
• Reactivity: Bovine
• Synonyms: Insulin-like 3 (INSL3); Bovine Insulin-like 3 (INSL3) ELISA Kit; INSL3 elisa kit

• Reactivity: Bovine

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INSL3 elisa kit

Intended Uses: This INS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat INS. This ELISA kit for research use only!

Principle of the Assay: INS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for INS. Standards or samples are then added to the microtiter plate wells and INS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of INS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for INS are added to each well to "sandwich" the INS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain INS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for INSL3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 317373369
• NCBI GeneID: 3640
• Molecular Weight: 16,872 Da
• NCBI Official Full Name: Insulin-like 3
• NCBI Official Synonym Full Names: insulin-like 3 (Leydig cell)
• NCBI Official Symbol: INSL3
• NCBI Official Synonym Symbols: RLF; RLNL; ley-I-L
• NCBI Protein Information: insulin-like 3; prepro-INSL3; relaxin-like factor b; leydig insulin-like peptide; leydig insulin -like hormone
• UniProt Protein Name: Insulin-like 3
• Protein Family: Insulin
• UniProt Gene Name: INSL3
• UniProt Synonym Gene Names: RLF; RLNL; Ley-I-L
• UniProt Entry Name: INSL3_HUMAN

NCBI Description

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2-p12

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: G-protein-coupled receptor binding; protease binding; hormone activity; insulin receptor binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; male gonad development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis

Disease: Cryptorchidism, Unilateral Or Bilateral