Pig inducible nitric oxide synthase ELISA Kit | iNOS elisa kit

Pig inducible nitric oxide synthase (iNOS) ELISA Kit

Cat. Num. AAA282359

• Gene Names: Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• Reactivity: Pig
• Synonyms: inducible nitric oxide synthase; Pig inducible nitric oxide synthase (iNOS) ELISA Kit; iNOS elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig NOS2. No significant cross-reactivity or interference between Pig NOS2 and analogues was observed.
• Sequence Length: 1147

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for iNOS elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NOS2 in samples. An antibody specific for NOS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 927028886
• NCBI GeneID: 18126
• NCBI Accession #: NP_001300850.1
• NCBI GenBank Nucleotide #: NM_001313921.1
• UniProt Accession #: P29477; O70515; O70516; Q5SXT3; Q6P6A0; Q8R410
• Molecular Weight: 130,575 Da
• NCBI Official Full Name: nitric oxide synthase, inducible isoform b
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inosl; Inducible NOS; iNOS; MAC-NOS

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 11 B5|11 46.74 cM

Cellular Component: cortical cytoskeleton; cytoplasm; cytosol; extracellular space; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome; vesicle membrane

Molecular Function: actin binding; arginine binding; beta-catenin binding; cadherin binding; calmodulin binding; cAMP-dependent protein kinase regulator activity; FAD binding; FMN binding; heme binding; Hsp90 protein binding; iron ion binding; metal ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; nitric-oxide synthase binding; oxidoreductase activity; protein binding; protein homodimerization activity; protein kinase binding; receptor binding; tetrahydrobiopterin binding

Biological Process: arginine catabolic process; cellular response to cytokine stimulus; cellular response to interferon-gamma; cellular response to organic cyclic compound; circadian rhythm; defense response to bacterium; inflammatory response; interleukin-6 secretion; interleukin-8 secretion; negative regulation of blood pressure; negative regulation of gene expression; negative regulation of protein catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; peptidyl-cysteine S-nitrosylation; positive regulation of blood vessel diameter; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; prostaglandin secretion; regulation of cell proliferation; regulation of cytokine production involved in inflammatory response; regulation of insulin secretion; response to hypoxia; response to lipopolysaccharide; superoxide metabolic process