Rabbit iNOS Polyclonal Antibody | anti-iNOS antibody

iNOS polyclonal antibody

Cat. Num. AAA565227

• Gene Names: Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• Reactivity: Mouse, Rat, Bovine
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: iNOS; Polyclonal Antibody; iNOS polyclonal antibody; NOS2; NOS II; Nitric oxide synthase (inducible); Hepatocyte NOS; anti-iNOS antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Bovine
• Clonality: Polyclonal
• Specificity: Recognizes mouse, rat, and bovine iNOS. Detects a band of ~130kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 1144

• Applicable Applications for anti-iNOS antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot (1:1,000, ECL); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~130kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis: rat brain extract, probed with iNOS, pAb.)

Related Product Information for anti-iNOS antibody

The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.

Product Categories/Family for anti-iNOS antibody

Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 266649
• NCBI GeneID: 18126
• NCBI Accession #: P29477.1
• NCBI GenBank Nucleotide #: NM_010927
• UniProt Accession #: P29477; O70515; O70516; Q5SXT3; Q6P6A0; Q8R410
• Molecular Weight: 130,575 Da
• NCBI Official Full Name: Nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inosl; Inducible NOS; iNOS; MAC-NOS
• UniProt Entry Name: NOS2_MOUSE

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39

Cellular Component: cortical cytoskeleton; cytoplasm; cytosol; extracellular space; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome; vesicle membrane

Molecular Function: actin binding; beta-catenin binding; cadherin binding; calmodulin binding; cAMP-dependent protein kinase regulator activity; FAD binding; FMN binding; heme binding; Hsp90 protein binding; iron ion binding; metal ion binding; nitric-oxide synthase activity; nitric-oxide synthase binding; oxidoreductase activity; protein binding; protein homodimerization activity; protein kinase binding; receptor binding

Biological Process: arginine catabolic process; defense response to bacterium; inflammatory response; negative regulation of blood pressure; negative regulation of protein catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of vasodilation; prostaglandin secretion; regulation of cell proliferation; regulation of insulin secretion; response to hypoxia; response to lipopolysaccharide; superoxide metabolic process