Nitric Oxide Synthase 2, Inducible (NOS2) Recombinant Protein | NOS2 recombinant protein

Recombinant Nitric Oxide Synthase 2, Inducible (NOS2)

Cat. Num. AAA2010129

• Gene Names: Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Nitric Oxide Synthase 2; Inducible (NOS2); Recombinant Nitric Oxide Synthase 2; NOS2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-SNN PTFLEVLEEF PSLHVPAAFL LSQLPILKPR YYSISSSQD
• Sequence Length: 1144

• Applicable Applications for NOS2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser868~Asp909 (Accession # P29477) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6754872
• NCBI GeneID: 18126
• NCBI Accession #: NP_035057.1
• NCBI GenBank Nucleotide #: NM_010927.3
• UniProt Accession #: P29477; O70515; O70516; Q5SXT3; Q6P6A0; Q8R410
• Molecular Weight: 6.3kDa
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNOS; Nos-2; Nos2a; i-NOS; NOS-II
• NCBI Protein Information: nitric oxide synthase, inducible; MAC-NOS; NOS type II; inducible NOS; macrophage NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2, inducible, macrophage
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inosl; Inducible NOS; iNOS; MAC-NOS
• UniProt Entry Name: NOS2_MOUSE

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; vesicle membrane; intracellular; cytosol; nucleus

Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; metal ion binding; beta-catenin binding; cAMP-dependent protein kinase regulator activity; oxidoreductase activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; protein binding; FMN binding; NADPH-hemoprotein reductase activity; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding

Biological Process: superoxide metabolic process; response to lipopolysaccharide; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; ovulation from ovarian follicle; positive regulation of vasodilation; regulation of cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of blood pressure; regulation of protein kinase activity; inflammatory response; nitric oxide biosynthetic process; negative regulation of protein catabolic process; nitric oxide mediated signal transduction; regulation of insulin secretion