ROCK Activity Immunoblot Kit

ROCK Activity Immunoblot Kit

Cat. Num. AAA168582

• Synonyms: ROCK Activity; ROCK Activity Immunoblot Kit; ROCK Activity immunoblot kit

• Preparation and Storage: Store active ROCK-II at -80 degree C and all other kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for ROCK Activity immunoblot kit

Background: Members of the Rho family are essential regulatory components of the signaling pathway that direct cell motility, adhesion, and cytokinesis through reorganization of actin cytoskeleton. Rho is activated by extracellular signals such as lysophosphatidic acid (LPA). The actions of Rho are mediated by downstream Rho effectors. One of these effectors is Rho-associated kinase (ROCK). Two ROCK isoforms have been identified: ROCK-I (also known as ROKbeta) and ROCK-II (also known as Rho Kinase and ROKalpha). ROCK mediates Rho signaling and reorganizes actin cytoskeleton through phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. For example, ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). ROCK Activity Immunoblot Kit utilizes recombinant MYPT1 as ROCK substrate. After incubating the substrate with ROCK samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated MYPT1 is detected by western blot analysis using an anti-phospho-MYPT1 (Thr696) (Figure 1). ROCK Activity Immunoblot Kit provides a simple and fast tool to monitor ROCK activity using its physiological substrate. The kit also includes active ROCK-II as a positive control. Each kit provides sufficient quantities to perform 20 assays.

Product Categories/Family for ROCK Activity immunoblot kit

Cell Signaling and Protein Biology; Kinase Assays; Rho Kinase (ROCK) Assays

References

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