RhoA Assay Kit

RhoA Activation Assay

Cat. Num. AAA169193

• Synonyms: RhoA; RhoA Activation Assay; RhoA Activation Assay Kit; RhoA assay kit

• Preparation and Storage: Store all kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaw cycles.

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Related Product Information for RhoA assay kit

Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. RhoA, a 21 kDa protein, regulating a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoA regulates molecular events by cycling between an inactive GDP-bound form and an active GTPbound form. In its active (GTP-bound) state, RhoA binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades. RhoA Activation Assay Kit utilizes Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoA specific monoclonal antibody (see Figure 3 and Assay Principle). RhoA Activation Assay Kit provides a simple and fast tool to monitor the activation of RhoA. The kit includes easily identifiable Rhotekin RBD Agarose beads (see Figure 1), pink in color, and a RhoA Immunoblot Positive Control for quick RhoA identification. Each kit provides sufficient quantities to perform 20 assays.

Product Categories/Family for RhoA assay kit

Cell Signaling and Protein Biology; G Protein Signaling/Small GTPase Assays and Vectors; Small GTPase Research Tools; Small GTPase Activation Assays

References

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