Rabbit ROCK-1 Monoclonal Antibody | anti-ROCK-1 antibody

ROCK-1 Rabbit Monoclonal Antibody

Cat. Num. AAA3014162

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: ROCK-1; Monoclonal Antibody; ROCK-1 Rabbit Monoclonal Antibody; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK; ROCK1; anti-ROCK-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of ROCK-1 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1354

• Applicable Applications for anti-ROCK-1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:50-1:200; ICC: 1:50-1:200; IF: 1:50-1:200; FC/FACS: 1:10-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ROCK-1 Rabbit mAb at 1:1000 dilutionLane1:A431 whole cell lysateLane2:NIH/3T3 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-ROCK-1 antibody

Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.

NCBI and Uniprot Product Information

• NCBI GI #: 47605999
• NCBI GeneID: 6093
• UniProt Accession #: Q13464
• Molecular Weight: ~ 158kDa
• NCBI Official Full Name: Rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho associated coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1
• UniProt Protein Name: Rho-associated protein kinase 1
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_HUMAN

NCBI Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: Golgi membrane; centriole; ruffle; cytoskeleton; lamellipodium; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; GTP-Rho binding; ATP binding; protein kinase activity

Biological Process: regulation of cell adhesion; axon guidance; apoptosis; bleb formation; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; leukocyte adhesion; smooth muscle contraction; positive regulation of focal adhesion formation; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; membrane to membrane docking; myoblast migration; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte tethering or rolling; regulation of keratinocyte differentiation; leukocyte migration; cell structure disassembly during apoptosis