Western Blot (WB)
(Western blot analysis of human Jurkat cells (lane 1), rat PC12 cells (lane 2), mouse brain tissue (lane 3), and mouse SYF fibroblasts (lane 4). The blot was probed with mouse monoclonal anti-ROCK-II (Central region) antibody at 1:1000 dilution (lanes 1-4).)
Mouse ROCK-II Monoclonal Antibody | anti-ROCK2 antibody
ROCK-II (Central region) Antibody
Gene Names
ROCK2; ROCK-II
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Purity
Protein A chromatography
Synonyms
ROCK-II; Monoclonal Antibody; ROCK-II (Central region) Antibody; ROCK-II (Central region); anti-ROCK2 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M276
Specificity
The antibody detects a 165kDa* band corresponding to ROCK-II in mouse kidney, heart, and brain tissues, as well as in rat PC12, mouse SYF, and human Jurkat cells.
Purity/Purification
Protein A chromatography
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-ROCK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
Clone (M276) was generated from amino acids in the central region of human ROCK-II. This sequence has high homology to rat and mouse ROCK-II, and has 50% homology to ROCK-I.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of human Jurkat cells (lane 1), rat PC12 cells (lane 2), mouse brain tissue (lane 3), and mouse SYF fibroblasts (lane 4). The blot was probed with mouse monoclonal anti-ROCK-II (Central region) antibody at 1:1000 dilution (lanes 1-4).)
Western Blot (WB)
(Western blot analysis of human Jurkat cells (lane 1), rat PC12 cells (lane 2), mouse brain tissue (lane 3), and mouse SYF fibroblasts (lane 4). The blot was probed with mouse monoclonal anti-ROCK-II (Central region) antibody at 1:1000 dilution (lanes 1-4).)
Related Product Information for anti-ROCK2 antibody
Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKbeta and ROCK-II/ROKalpha) includes an N-terminal catalytic domain, a coiled-coil region, and a C-terminal pleckstrin homology domain. The C-terminal region negatively regulates kinase activity, and this inhibitory regulation is controlled through C-terminal cleavage. Caspase-3-induced cleavage of ROCK-I and direct cleavage of ROCK-II by granzyme B activate ROCK. This leads to inhibition of myosin phosphatase and phosphorylation of myosin light chain, which causes myosin II-mediated changes in cytoskeletal dynamics. This pathway has been implicated as a downstream effector of Rho-mediated changes in cytokinesis, cell motility, apoptosis and muscle contraction.
References
Sebbagh, M. et al. (2005) J. Exp. Med. 201:465.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,800 Da
NCBI Official Full Name
rho-associated protein kinase 2
NCBI Official Symbol
ROCK2
NCBI Official Synonym Symbols
ROCK-II
NCBI Protein Information
rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II
UniProt Protein Name
Rho-associated protein kinase 2
Protein Family
UniProt Gene Name
ROCK2
UniProt Synonym Gene Names
ROCK-II
UniProt Entry Name
ROCK2_BOVIN
Similar Products
Product Notes
The ROCK2 rock2 (Catalog #AAA474548) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ROCK-II (Central region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ROCK-II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the ROCK2 rock2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROCK-II, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
