Western Blot (WB)
(Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-ROCK-1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates ROCK-1 (~ 158 kDa).)
Rabbit anti-Human, Mouse ROCK-1 Polyclonal Antibody | anti-rok antibody
ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1)
Gene Names
rok; CG9774; DmelCG9774; DRhk; drok; dRok; dROK; Drok; DRok; DROK; Rhk; RhoK; Rock; ROCK; ROCK-1; ROCK1; Rok; ROK; ROKalpha/beta
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ROCK-1; Polyclonal Antibody; ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1; ROCK1; MGC131603; MGC43611; NY-REN-35 Antigen; p160 ROCK1; p160ROCK; Renal Carcinoma Antigen NY-REN-35; Rho-associated Protein Kinase 1); Anti -ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1; anti-rok antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes human ROCK-1. Species Crossreactiviy: mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM socium chloride, 0.05% sodium azide.
Applicable Applications for anti-rok antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500
Dilution: Western Blot: 1:500
Immunogen
Synthetic peptide corresponding to human ROCK-1.
Positive Control
A431 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-ROCK-1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates ROCK-1 (~ 158 kDa).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-ROCK-1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates ROCK-1 (~ 158 kDa).)
Related Product Information for anti-rok antibody
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROK alpha/ROCK-II and ROK beta/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. The serine-threonine kinase ROCK-1 is a down-stream effector of Rho, and is involved in signal transduction pathways that regulate cell morphology, motility, and tumor progression through the reorganization of the actin cytoskeleton.
Product Categories/Family for anti-rok antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,307 Da
NCBI Official Full Name
Rho-kinase
NCBI Official Synonym Full Names
Rho-kinase
NCBI Official Symbol
rok
NCBI Official Synonym Symbols
CG9774; DmelCG9774; DRhk; drok; dRok; dROK; Drok; DRok; DROK; Rhk; RhoK; Rock; ROCK; ROCK-1; ROCK1; Rok; ROK; ROKalpha/beta
NCBI Protein Information
CG9774 gene product from transcript CG9774-RA; CG9774-PA; DRho-kinase; ROCK; Rho-associated kinase; RhoA kinase; drosophila rho-associated kinase; rho kinase; rok-PA
UniProt Protein Name
FI04037p
Protein Family
UniProt Gene Name
rok
UniProt Synonym Gene Names
rok-RA
UniProt Entry Name
Q9VXE3_DROME
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein. SAAS SAAS002219
Sequence similarities: Contains AGC-kinase C-terminal domain. SAAS SAAS002219Contains PH domain. SAAS SAAS002219Contains protein kinase domain. SAAS SAAS002219
Research Articles on rok
Similar Products
Product Notes
The rok rok (Catalog #AAA630721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ROCK-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:500. Researchers should empirically determine the suitability of the rok rok for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROCK-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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