IL-22BP ELISA Pair Set | IL22RA2 elisa pair set

IL-22BP Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118337

• Synonyms: IL-22BP; IL-22BP Matched ELISA Antibody Pair Set; Human; Human IL-22BP/IL-22RA2 ELISA Pair Set; interleukin 22 receptor; alpha 2; CRF2-10 Matched ELISA Antibody Pair Set; CRF2-S1 Matched ELISA Antibody Pair Set; CRF2X Matched ELISA Antibody Pair Set; IL-22R-alpha-2 Matched ELISA Antibody Pair Set; IL-22RA2 Matched ELISA Antibody Pair Set; ZCYTOR16 Matched ELISA Antibody Pair Set; IL22RA2 elisa pair set

• Specificity: Quantitative determination of Human IL-22BP

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 132.81-8500pg/mL
• Sensitivity: 132.81pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human IL-22BP/IL-22RA2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human IL-22BP/IL-22RA2 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 585 ng of recombinant Human IL-22BP/IL-22RA2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for IL22RA2 elisa pair set

Interleukin-22 receptor subunit alpha-2 (IL-22RA2), also known as interleukin-22-binding protein (IL-22BP), is a subunit of the receptor for interleukin 22. IL-22BP belongs to the type II cytokine receptor family and contains 3 fibronectin type-III domains. IL-22BP/IL-22RA2 is expressed in a range of tissues, including those in the digestive, female reproductive, and immune systems. It is expressed in the placenta, spleen, breast, skin, and lung. It is also detected in the intestinal tract, testis, brain, heart, and thymus. The dominant cell types expressing IL-22BP/IL-22RA2 were mononuclear cells and epithelium. IL-22BP/IL-22RA2 may play an important role as an IL-22 antagonist in the regulation of inflammatory responses. Interleukin-22 (IL-22) is a member of the IL-10 family. It is produced by T cells and induces the production of acute-phase reactants. IL-22 plays important role in the immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptors have been discovered, a membrane-bound receptor and a soluble receptor.

This IL-22BP Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL-22BP. It contains Human IL-22BP capture antibody, Human IL-22BP detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL-22BP protein.

References

Whittington HA, et al. (2004) Interleukin-22: a potential immunomodulatory molecule in the lung. Am J Respir Cell Mol Biol. 31(2): 220-6.Dumoutier L, et al. (2001) Cloning and characterization of IL-22 binding protein, a natural antagonist of IL-10-related T cell-derived inducible factor/IL-22. J Immunol. 166(12): 7090-5.Wei CC, et al. (2003) Cloning and characterization of mouse IL-22 binding protein. Genes Immun. 4(3): 204-11.