Western Blot/Blotting
(Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X1848P (1 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce?s Super Signal West Femto.)
Rabbit Sphingosine Kinase 1, Long Form, Rat Reactive Polyclonal Antibody | anti-Sphk1 antibody
Sphingosine Kinase 1, Long Form, Rat Reactive
Western Blot/Blotting
(Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X1848P (1 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce?s Super Signal West Femto.)
Western Blot/Blotting
(Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X1848P (1 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce?s Super Signal West Femto.)
NCBI and Uniprot Product Information
Uniprot Description
SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91
Chromosomal Location of Human Ortholog: 17q25.2
Cellular Component: synaptic vesicle; axon; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: D-erythro-sphingosine kinase activity; calmodulin binding; protein binding; DNA binding; protein phosphatase 2A binding; sphinganine kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: protein folding; positive regulation of NF-kappaB import into nucleus; female pregnancy; signal transduction; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; activation of NF-kappaB transcription factor; 'de novo' posttranslational protein folding; response to magnesium ion; positive regulation of fibroblast proliferation; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of smooth muscle contraction; inflammatory response; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; cyclooxygenase pathway; calcium-mediated signaling; sphingosine metabolic process; lipid phosphorylation; cellular response to starvation; positive regulation of cell growth; response to ATP; positive regulation of angiogenesis; cellular protein metabolic process; positive regulation of protein ubiquitination; positive regulation of protein amino acid phosphorylation; brain development; vascular endothelial growth factor receptor signaling pathway; response to progesterone stimulus; negative regulation of apoptosis; sphingoid catabolic process; positive regulation of cell migration; response to amine stimulus
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Product Notes
The Sphingosine Kinase 1, Long Form, Rat Reactive sphk1 (Catalog #AAA395566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sphingosine Kinase 1, Long Form, Rat Reactive reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Sphingosine Kinase 1, Long Form, Rat Reactive can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Sphingosine Kinase 1, Long Form, Rat Reactive sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine Kinase 1, Long Form, Rat Reactive, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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