Rabbit anti-Human, Mouse WTX Polyclonal Antibody | anti-WTX antibody

WTX, ID (Protein FAM123B, Wilms Tumor Gene On The X Chromosome Protein, FAM123B) (MaxLight 650)

Cat. Num. AAA6506012

• Gene Names: AMER1; WTX; OSCS; FAM123B
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: WTX; Polyclonal Antibody; ID (Protein FAM123B; Wilms Tumor Gene On The X Chromosome Protein; FAM123B) (MaxLight 650); anti-WTX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WTX. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 1135

• Applicable Applications for anti-WTX antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human WTX (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of WTX antibody (Center) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(WTX Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WTX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WTX Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-WTX antibody

Antibodies; Cancer Markers

References

1. Rivera, M.N., Proc. Natl. Acad. Sci. USA 106(20), 8338-8343 (2009). 2. Fukuzawa, R., Oncogene 28(8), 1063-1075 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 124244056
• NCBI GeneID: 139285
• NCBI Accession #: NP_689637
• NCBI GenBank Nucleotide #: NP_689637.3
• UniProt Accession #: Q5JTC6
• Molecular Weight: 124.029
• NCBI Official Full Name: APC membrane recruitment protein 1
• NCBI Official Synonym Full Names: APC membrane recruitment protein 1
• NCBI Official Symbol: AMER1
• NCBI Official Synonym Symbols: WTX; OSCS; FAM123B
• NCBI Protein Information: APC membrane recruitment protein 1
• UniProt Protein Name: APC membrane recruitment protein 1
• UniProt Gene Name: AMER1
• UniProt Synonym Gene Names: FAM123B; WTX; Amer1
• UniProt Entry Name: AMER1_HUMAN

NCBI Description

The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]

Uniprot Description

WTX: a peripheral membrane protein on the cytoplasmic side that shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Enhances trancription activation by WT1. Promotes CTNNB1 ubiquitination and degradation. Antagonizes Wnt and CTNNB1 signaling. Inactivated in approximately one-third of Wilms tumors. Detected in fetal and adult kidney, brain and spleen. Involved in kidney development. Interacts with CTNNB1, AXIN1, KEAP1, APC and BTRC. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in a complex containing CTNNB1, APC, AXIN1 and AXIN2. Two alternatively spliced human isoforms have been reported.

Chromosomal Location of Human Ortholog: Xq11.2

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; beta-catenin binding

Biological Process: Wnt receptor signaling pathway

Disease: Osteopathia Striata With Cranial Sclerosis