IL-2 active protein

IL-2

Cat. Num. AAA515796

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Applications: Cell Assay
• Synonyms: IL-2; TCGF; lymphokine; IL2; IL-2 active protein

• Host: E Coli
• Form/Format: Recombinant protein stored in 1 X PBS, 10% glycerol.

• Applicable Applications for IL-2 active protein: Cell Assay
• Species: Human
• Endotoxin Level: Less than 0.1ng/ug (IEU/ug) of IL-2
• Preparation and Storage: Store at desiccated below -20 degree C. It is stable at 4 degree C between 2-7 days. For long term storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Activity Assay: Sample Biological Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for IL-2 active protein

Human active form IL-2 (21-153) was expressed in E Coli cells and purified by proprietary chromatographic techniques. The protein accession number is NP_000577.2
IL-2 or interleukin 2 is a secreted cytokine which referred to as T-cell growth factor, that is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. IL2 can act as a growth hormone that is important for the proliferation of T and B-lymphocytes (1). IL2 expression is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene (2).

Product Categories/Family for IL-2 active protein

Growth Factors; AKT/PKB Pathway; ERK/MAPK Pathway; Inflammation; JAK/STAT Pathway; JNK/SAPK Pathway; NfkB Pathway; p38 Pathway; PKA/PKC Pathway

References

1. Lowenthal, J. W. et.al: Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985.
2. Hollander, G. A. et.al: Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• UniProt Accession #: P60568; P01585
• Molecular Weight: 17,628 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Oncoprotein; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; glycosphingolipid binding; carbohydrate binding; kappa-type opioid receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; negative regulation of protein amino acid phosphorylation; protein kinase C activation; positive regulation of cell proliferation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis