Rabbit POLR2J Polyclonal Antibody | anti-POLR2J antibody

POLR2J Rabbit Polyclonal

Cat. Num. AAA7602859

• Gene Names: POLR2J; RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: POLR2J; Polyclonal Antibody; POLR2J Rabbit Polyclonal; anti-POLR2J antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 117

• Applicable Applications for anti-POLR2J antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart using MBS7602859 (POLR2J antibody) at dilution of 1:100)

Western Blot (WB)

(A549 cells were subjected to SDS PAGE followed by western blot with MBS7602859 (POLR2J antibody) at dilution of 1:800)

NCBI and Uniprot Product Information

• NCBI GI #: 146327795
• NCBI GeneID: 5439
• Molecular Weight: 13,293 Da
• NCBI Official Full Name: POLR2J protein
• NCBI Official Synonym Full Names: polymerase (RNA) II subunit J
• NCBI Official Symbol: POLR2J
• NCBI Official Synonym Symbols: RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB11-a
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB11-a
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2J
• UniProt Synonym Gene Names: POLR2J1; RNA polymerase II subunit B11-a; RPB11a
• UniProt Entry Name: RPB11_HUMAN

NCBI Description

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Protein type: RNA processing; Transferase; DNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA-directed RNA polymerase activity; LRR domain binding; protein binding; protein dimerization activity

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair