Typical Testing Data/Standard Curve (for reference only)
Mouse interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) ELISA Kit | IL12B elisa kit
Mouse interleukin 12, IL-12/P40 ELISA Kit
Gene Names
Il12b; p40; Il-12b; Il12p40; Il-12p40
Reactivity
Mouse
Synonyms
interleukin 12B (natural killer cell stimulatory factor 2; cytotoxic lymphocyte maturation factor 2; p40); Mouse interleukin 12; IL-12/P40 ELISA Kit; IL-12/P40 ELISA KIT; CLMF; CLMF2; IL-12B; NKSF; NKSF2; IL12; subunit p40; interleukin 12; p40; interleukin 12B; interleukin-12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; IL12B elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse IL-12/P40. No significant cross-reactivity or interference between mouse IL-12/P40 and analogues was observed.
Sequence Length
335
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
6.25 pg/ml-400 pg/ml.
Sensitivity
The minimum detectable dose of mouse IL-12/P40 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL12B elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12/P40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12/P40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12/P40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12/P40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,235 Da
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12b
NCBI Official Symbol
Il12b
NCBI Official Synonym Symbols
p40; Il-12b; Il12p40; Il-12p40
NCBI Protein Information
interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit
UniProt Protein Name
Interleukin-12 subunit beta
Protein Family
UniProt Gene Name
Il12b
UniProt Synonym Gene Names
IL-12B; CLMF p40
UniProt Entry Name
IL12B_MOUSE
NCBI Description
This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]
Uniprot Description
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC 1 PublicationManual assertion based on experiment in:Ref.4
Research Articles on IL12B
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Product Notes
The Mouse IL12B il12b (Catalog #AAA704150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704150 ELISA Kit recognizes Mouse IL12B. It is sometimes possible for the material contained within the vial of "interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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