Typical Testing Data/Standard Curve
Goat Interleukin 12 p40 ELISA Kit | IL-12 p40 elisa kit
Goat Interleukin 12 p40 ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||IL-12 p40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-12 p40 antibody and an IL-12 p40-HRP conjugate. The assay sample and buffer are incubated together with IL-12 p40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12 p40 concentration since IL-12 p40 from samples and IL-12 p40-HRP conjugate compete for the anti-IL-12 p40 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12 p40 from the sample, fewer sites are left to bind IL-12 p40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12 p40 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]
Uniprot Description
IL12B: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 3 subfamily.
Protein type: Secreted; Cytokine; Secreted, signal peptide
Cellular Component: extracellular space; membrane; interleukin-12 complex; cytoplasm; extracellular region
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; interleukin-12 alpha subunit binding; protein homodimerization activity; growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein heterodimerization activity; cytokine activity; interleukin-23 receptor binding
Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of cell adhesion; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of tyrosine phosphorylation of Stat4 protein; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; sensory perception of pain; positive regulation of natural killer cell activation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; cell surface receptor linked signal transduction; positive regulation of lymphocyte proliferation; natural killer cell activation during immune response; positive regulation of T cell proliferation; negative regulation of inflammatory response to antigenic stimulus; cell cycle arrest; defense response to virus; positive regulation of interleukin-17 production; positive regulation of NK T cell activation; cell migration; positive regulation of natural killer cell proliferation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; T-helper cell differentiation; positive regulation of tumor necrosis factor production; defense response to protozoan; regulation of tyrosine phosphorylation of Stat1 protein; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of defense response to virus by host
Research Articles on IL-12 p40
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Product Notes
The Goat IL-12 p40 il12b (Catalog #AAA747364) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA747364 ELISA Kit recognizes Goat IL-12 p40. It is sometimes possible for the material contained within the vial of "Interleukin 12 p40, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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