Chicken IL-12 p40 ELISA Kit | IL-12 p40 elisa kit
Chicken IL-12 p40 (Interleukin 12 p40) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IgY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ChickenIgY and Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessivelyto each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken IgY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of ChickenIgY. You can calculate the concentration of Chicken IgY in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]
Uniprot Description
IL12B: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 3 subfamily.
Protein type: Cytokine; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; membrane; interleukin-12 complex; cytoplasm; extracellular region
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; interleukin-12 alpha subunit binding; protein binding; protein homodimerization activity; growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein heterodimerization activity; interleukin-23 receptor binding; cytokine activity
Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of cell adhesion; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of tyrosine phosphorylation of Stat4 protein; sensory perception of pain; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; positive regulation of natural killer cell activation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; cell surface receptor linked signal transduction; positive regulation of lymphocyte proliferation; natural killer cell activation during immune response; negative regulation of inflammatory response to antigenic stimulus; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; positive regulation of interleukin-17 production; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; cell migration; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; T-helper cell differentiation; positive regulation of tumor necrosis factor production; defense response to protozoan; regulation of tyrosine phosphorylation of Stat1 protein; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of mononuclear cell proliferation; positive regulation of defense response to virus by host
