Sheep Interleukin 12 p40 ELISA Kit | IL-12 p40 elisa kit

Sheep Interleukin 12 p40 ELISA Kit

Cat. Num. AAA744779

• Gene Names: Il12b; p40; Il-12b; Il12p40; Il-12p40
• Reactivity: Sheep
• Synonyms: Interleukin 12 p40; Sheep Interleukin 12 p40 ELISA Kit; IL-12 p40 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This IL-12 p40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-12 p40. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This IL-12 p40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-12 p40. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-12 p40 elisa kit

Principle of the Assay: IL-12 p40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-12 p40 antibody and an IL-12 p40-HRP conjugate. The assay sample and buffer are incubated together with IL-12 p40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12 p40 concentration since IL-12 p40 from samples and IL-12 p40-HRP conjugate compete for the anti-IL-12 p40 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12 p40 from the sample, fewer sites are left to bind IL-12 p40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12 p40 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-12 p40 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 6680397
• NCBI GeneID: 16160
• NCBI Accession #: NP_032378.1
• NCBI GenBank Nucleotide #: NM_008352.2
• Molecular Weight: 38,235 Da
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12b
• NCBI Official Symbol: Il12b
• NCBI Official Synonym Symbols: p40; Il-12b; Il12p40; Il-12p40
• NCBI Protein Information: interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit
• UniProt Protein Name: Interleukin-12 subunit beta
• UniProt Gene Name: Il12b
• UniProt Synonym Gene Names: IL-12B; CLMF p40
• UniProt Entry Name: IL12B_MOUSE

NCBI Description

This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]

Uniprot Description

IL12B: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Cellular Component: extracellular space; membrane; interleukin-12 complex; cytoplasm; extracellular region

Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-12 alpha subunit binding; protein homodimerization activity; growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein heterodimerization activity; interleukin-23 receptor binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of cell adhesion; positive regulation of T-helper 1 type immune response; negative regulation of interleukin-10 production; positive regulation of T cell mediated cytotoxicity; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; natural killer cell activation; sensory perception of pain; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; positive regulation of natural killer cell activation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; cell surface receptor linked signal transduction; positive regulation of lymphocyte proliferation; natural killer cell activation during immune response; positive regulation of T cell proliferation; negative regulation of inflammatory response to antigenic stimulus; cell cycle arrest; defense response to virus; positive regulation of interleukin-17 production; cell migration; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; T-helper cell differentiation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; defense response to protozoan; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of defense response to virus by host