Mouse IL-10 ELISA Kit | IL10 elisa kit

Mouse IL-10 PicoKine ELISA Kit

Cat. Num. AAA175883

• Gene Names: Il10; CSIF; Il-10
• Reactivity: Mouse
• Synonyms: IL-10; Mouse IL-10 PicoKine ELISA Kit; Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF; IL10 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il10
• Sequence Length: 178

• Samples: Cell culture supernatants and serum
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <1 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL10 elisa kit

Principle of the Assay: The Picokine™ Mouse Il10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il10 with a 96-well strip plate that is pre-coated with antibody specific for Il10. The detection antibody is a biotinylated antibody specific for Il10. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il10 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S19-S178. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il10, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Il10 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il10 in the sample.

Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 408360145
• NCBI GeneID: 16153
• UniProt Accession #: P18893; Q0VBJ1
• Molecular Weight: 20,641 Da
• NCBI Official Full Name: Interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: CSIF; Il-10
• NCBI Protein Information: interleukin-10; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF
• UniProt Entry Name: IL10_MOUSE

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity; interleukin-10 receptor binding

Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; negative regulation of interferon-gamma production; positive regulation of MHC class II biosynthetic process; response to molecule of bacterial origin; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor production; negative regulation of cytokine secretion during immune response; regulation of gene expression; negative regulation of inflammatory response; negative regulation of cytokine production; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; receptor biosynthetic process; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion