Rabbit anti-Human, Mouse Myosin Light Chain Kinase 3 Polyclonal Antibody | anti-MYLK3 antibody

Myosin Light Chain Kinase 3, NT (MYLK3, MLCK2, MLCK, caMLCK, Cardiac-MyBP-C-associated Ca/CaM Kinase, MGC126319, MGC126320, Putative Myosin Light Chain Kinase 3) (Azide free) (HRP)

Cat. Num. AAA6489134

• Gene Names: MYLK3; MLCK; MLCK2; caMLCK
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Myosin Light Chain Kinase 3; Polyclonal Antibody; NT (MYLK3; MLCK2; MLCK; caMLCK; Cardiac-MyBP-C-associated Ca/CaM Kinase; MGC126319; MGC126320; Putative Myosin Light Chain Kinase 3) (Azide free) (HRP); EC=2.7.11.18; anti-MYLK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLCK3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MYLK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 40-69 in the N-terminal region of human MLCK3., UniProt Accession #Q32MK0; Q96DV1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A375 cell line lysates (35ug/lane) usingMBS628238. MYLK3 (arrow) was detected usingMBS628238.)

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS628238. MYLK3 (arrow) was detected usingMBS628238.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS628238, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted withMBS628238, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Related Product Information for anti-MYLK3 antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue-nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Product Categories/Family for anti-MYLK3 antibody

Antibodies; Myosin

References

1. Blume-Jensen P, et al. Nature 2001. 411: 355. 2. Cantrell D, J. Cell Sci. 2001. 114: 1439. 3. Jhiang S Oncogene 2000. 19: 5590. 4. Manning G, et al. Science 2002. 298: 1912. 5. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. 6.R obertson, S. et al. Trends Genet. 2000. 16: 368. 7. Robinson D, et al. Oncogene 2000. 19: 5548. 8. Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. 9. Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. 10.Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

NCBI and Uniprot Product Information

• NCBI GI #: 815930021
• NCBI GeneID: 91807
• NCBI Accession #: NP_001295230.1
• NCBI GenBank Nucleotide #: NM_001308301.1
• UniProt Accession #: Q96DV1; Q32MK1; B5BUL9; B7Z5U8; Q32MK0
• Molecular Weight: 52,723 Da
• NCBI Official Full Name: myosin light chain kinase 3 isoform 2
• NCBI Official Synonym Full Names: myosin light chain kinase 3
• NCBI Official Symbol: MYLK3
• NCBI Official Synonym Symbols: MLCK; MLCK2; caMLCK
• NCBI Protein Information: myosin light chain kinase 3
• UniProt Protein Name: Myosin light chain kinase 3
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: MYLK3
• UniProt Synonym Gene Names: MLCK; Cardiac-MLCK

NCBI Description

Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]

Uniprot Description

caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CAMK group; EC 2.7.11.18; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16q11.2

Cellular Component: cytoplasm; cytosol; intracellular

Molecular Function: calmodulin-dependent protein kinase activity; myosin light chain kinase activity

Biological Process: cardiac myofibril assembly; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; regulation of vascular permeability during acute inflammatory response; sarcomere organization; sarcomerogenesis