Human IL-10 ELISA Kit | IL10 elisa kit

Human IL-10 ELISA Kit

Cat. Num. AAA8244574

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL-10; Human IL-10 ELISA Kit; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF; IL10 elisa kit

• Reactivity: Human
• Specificity: The Human IL-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-10 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 178

• Applicable Applications for IL10 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
• Sensitivity: 7 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for IL10 elisa kit

Principle of the Assay||The Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

NCBI and Uniprot Product Information

• NCBI GI #: 124292
• NCBI GeneID: 3586
• UniProt Accession #: P22301
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: Interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF
• UniProt Entry Name: IL10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; interleukin-10 receptor binding; protein binding

Biological Process: aging; B cell differentiation; B cell proliferation; cell-cell signaling; cytoplasmic sequestering of NF-kappaB; defense response to bacterium; defense response to protozoan; hemopoiesis; inflammatory response; leukocyte chemotaxis; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; negative regulation of cytokine secretion during immune response; negative regulation of interferon-alpha biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-1 production; negative regulation of interleukin-12 production; negative regulation of interleukin-18 production; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of MHC class II biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of T cell proliferation; negative regulation of tumor necrosis factor biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptosis; positive regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; receptor biosynthetic process; regulation of gene expression; regulation of isotype switching; regulation of sensory perception of pain; response to activity; response to drug; response to glucocorticoid stimulus; response to inactivity; response to insulin stimulus; response to molecule of bacterial origin; T-helper 2 type immune response

Disease: Graft-versus-host Disease, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Rheumatoid Arthritis